DNA topoisomerase II alpha (TOP2A) Rabbit Polyclonal Antibody (CAB0726)
The Anti-DNA Topoisomerase II Alpha (TOP2A) Antibody (CAB0726) is a vital tool for researchers studying the role of TOP2A in DNA replication and repair processes. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an excellent choice for Western blot analysis.TOP2A is a key enzyme involved in DNA unwinding and relaxation, essential for cell division and chromosome segregation. Dysregulation of TOP2A has been implicated in various cancers, making it a promising target for cancer therapy research.
The Anti-TOP2A Antibody binds specifically to the TOP2A protein, enabling precise detection and analysis in a variety of cell types.By understanding the function of TOP2A and its role in DNA processes, researchers can uncover novel insights into cancer biology and potential therapeutic strategies. The Anti-DNA Topoisomerase II Alpha Antibody is a valuable tool for advancing research in cancer treatment and personalized medicine.
Product Name:
DNA topoisomerase II alpha (TOP2A) Rabbit Polyclonal Antibody
SKU:
CAB0726
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1314-1503 of human DNA topoisomerase II alpha (TOP2A) (NP_001058.2).
TOP2; TP2A; TOPIIA; TOP2alpha; DNA topoisomerase II alpha (TOP2A)
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleoplasm.
Calculated MW:
174kDa
Observed MW:
Refer to figures
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Purification Method:
Affinity purification
Gene ID:
7153
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunofluorescence analysis of U-2 OS cells using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) Rabbit pAb (CAB0726) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
