The TOP1MT Polyclonal Antibody (CAB15559) is a valuable tool for researchers investigating the TOP1MT protein, which is involved in mitochondrial DNA replication and repair. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting TOP1MT in human samples, making it ideal for use in Western blotting and immunohistochemistry applications.TOP1MT is a key enzyme in the maintenance of mitochondrial genome integrity, playing a crucial role in mitochondrial DNA replication and repair processes. Dysregulation of TOP1MT has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular function and disease pathology.
By studying TOP1MT expression and activity, researchers can gain insights into mitochondrial dysfunction and its implications in disease development.Furthermore, the TOP1MT Polyclonal Antibody enables researchers to explore the biological functions of TOP1MT in different cell types and conditions, paving the way for advancements in mitochondrial research and potential therapeutic strategies targeting mitochondrial DNA maintenance. Its reliability and versatility make it a valuable asset for studies focusing on mitochondrial biology and its role in human health and disease.
Product Name:
TOP1MT Rabbit Polyclonal Antibody
SKU:
CAB15559
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1).
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
116447
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using TOP1MT Rabbit pAb (CAB15559) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60S.
