DNA topoisomerase I (TOP1) Rabbit Monoclonal Antibody (CAB12409)
The TOP1 Rabbit Monoclonal Antibody is a cutting-edge tool for researchers studying TOP1, an essential enzyme involved in DNA replication and transcription processes. This highly specific antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot and immunohistochemistry applications.TOP1 plays a crucial role in maintaining genomic stability and is a known target for cancer therapies, making it a key focus in cancer research. By targeting TOP1, researchers can gain valuable insights into the mechanisms underlying cancer development and potentially identify novel therapeutic strategies.
The TOP1 Rabbit Monoclonal Antibody enables precise detection and analysis of TOP1 protein expression in various cell types, providing researchers with a powerful tool for investigating the role of TOP1 in cellular processes. Its versatility and reliability make it an essential component in studies exploring DNA replication, gene transcription, and cancer biology.
Product Name:
DNA topoisomerase I (TOP1) Rabbit Monoclonal Antibody
SKU:
CAB12409
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TOPI; DNA topoisomerase I (TOP1)
Positive Sample:
Mouse thymus,HeLa,MCF7,NIH/3T3,K-562
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
91kDa
Observed MW:
100kDa
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Purification Method:
Affinity purification
Gene ID:
7150
Clone Number:
ARC0708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNA topoisomerase I (DNA topoisomerase I (TOP1)) Rabbit mAb (CAB12409) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
