DNA topoisomerase I (TOP1) Rabbit Polyclonal Antibody (CAB12524)
The TOP1 Polyclonal Antibody (CAB12524) is a valuable tool for researchers investigating the TOP1 protein, an essential enzyme involved in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the TOP1 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for studies in molecular biology and cancer research.
TOP1 plays a crucial role in DNA unwinding and rejoining during DNA replication and transcription, making it a key target for anticancer therapies. Dysregulation of TOP1 has been linked to increased genomic instability and carcinogenesis, highlighting its importance in cancer biology. By studying the activity of TOP1, researchers can gain insights into the mechanisms underlying cancer development and potentially identify novel therapeutic targets for cancer treatment.
Product Name:
DNA topoisomerase I (TOP1) Rabbit Polyclonal Antibody
SKU:
CAB12524
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1).
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Purification Method:
Affinity purification
Gene ID:
7150
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNA topoisomerase I (TOP1) Rabbit pAb (CAB12524) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
