The TNNT3 Polyclonal Antibody (CAB15323) is a valuable tool for researchers studying the TNNT3 protein, which is a key component of the troponin complex involved in muscle contraction. This antibody, produced in rabbits, exhibits high specificity and sensitivity when used with human samples, making it a reliable choice for Western blotting and immunohistochemistry experiments.TNNT3 is critical for proper muscle function, and mutations in this gene have been associated with various muscle disorders and cardiomyopathies. By targeting TNNT3 with this antibody, researchers can investigate its role in muscle physiology and disease development.
This antibody's versatility allows for the visualization and analysis of TNNT3 expression in different cell types and tissues, providing valuable insights into muscle-related conditions.With its ability to specifically bind to TNNT3, this polyclonal antibody is a powerful tool for advancing our understanding of muscle biology and identifying potential therapeutic targets for muscle-related disorders. Its reliable performance and proven effectiveness in research make it an essential component for any study involving TNNT3.
Product Name:
TNNT3 Rabbit Polyclonal Antibody
SKU:
CAB15323
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 147-256 of human TNNT3 (NP_001036246.1).
The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
Purification Method:
Affinity purification
Gene ID:
7140
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TNNT3 Rabbit pAb (CAB15323) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
