The TNNT1 Polyclonal Antibody (CAB10354) is a valuable tool for research involving TNNT1, a protein involved in muscle contraction. This antibody, generated in rabbits, has high specificity and sensitivity towards human samples, making it ideal for Western blot applications. It specifically binds to the TNNT1 protein, enabling researchers to detect and analyze its expression levels in various cell types.TNNT1, also known as Troponin T Type 1, is a crucial component of the troponin complex, which regulates muscle contraction in response to calcium signaling.
Mutations in the TNNT1 gene have been linked to muscle disorders such as nemaline myopathy, highlighting its importance in muscle function and disease.Studying TNNT1 with the CAB10354 antibody can provide valuable insights into muscle biology, muscle disorders, and potential therapeutic targets for muscle-related diseases. This antibody is a reliable tool for researchers in the fields of muscle physiology, neuromuscular disorders, and drug development.
Product Name:
TNNT1 Rabbit Polyclonal Antibody
SKU:
CAB10354
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human TNNT1 (NP_001119604.1).
This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7138
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TNNT1 antibody (CAB10354) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
