The cTnI Polyclonal Antibody (CAB6995) is a valuable tool for researchers studying cardiac troponin I (cTnI), a key protein involved in cardiac muscle contraction. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to cTnI, this antibody allows for precise detection and analysis in a variety of cell types, making it essential for studies in cardiology and heart disease research.
cTnI is a crucial biomarker for diagnosing and monitoring heart conditions such as myocardial infarction (heart attack) and heart failure. Understanding the regulation and function of cTnI is essential for developing new diagnostic tools and therapies to improve patient outcomes in cardiovascular diseases. The cTnI Polyclonal Antibody provides researchers with a reliable and accurate tool to study the role of cTnI in cardiac health and disease.
Product Name:
TNNI3 Rabbit Polyclonal Antibody
SKU:
CAB6995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cardiac Troponin I (TNNI3) (NP_000354.4).
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3)
Positive Sample:
Mouse heart,Rat heart,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
cardiac myofibril, cytosol, sarcomere
Calculated MW:
24kDa
Observed MW:
26kDa
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.
Purification Method:
Affinity purification
Gene ID:
7137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cardiac Troponin I (TNNI3) antibody (CAB6995) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
