The TNNT3 Polyclonal Antibody (CAB0152) is a valuable tool for researchers studying troponin T type 3 (TNNT3), a protein found in cardiac muscle tissue. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blot applications. By binding specifically to the TNNT3 protein, researchers can accurately detect and analyze its expression in various cell types, aiding in the study of heart function and cardiovascular diseases.TNNT3 is a crucial component of the troponin complex, which regulates muscle contraction in the heart. Mutations in TNNT3 have been linked to various heart conditions, making it a potential target for therapeutic intervention.
By understanding the role of TNNT3 in cardiac muscle function, researchers can gain insights into the mechanisms of heart disease and potentially develop new treatments for related disorders.Overall, the TNNT3 Polyclonal Antibody (CAB0152) offers researchers a reliable tool for investigating the function of TNNT3 in cardiac muscle and its implications for cardiovascular health. Its high reactivity and specificity make it a valuable asset for studies aimed at uncovering the molecular mechanisms underlying heart diseases.
Product Name:
TNNI3 Rabbit Polyclonal Antibody
SKU:
CAB0152
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human Cardiac Troponin I (TNNI3) (NP_000354.4).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3)
Positive Sample:
Mouse heart,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
cardiac myofibril, cytosol, sarcomere
Calculated MW:
24kDa
Observed MW:
28kDa
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.
Purification Method:
Affinity purification
Gene ID:
7137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit pAb (CAB0152) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
