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Anti-TNK2 Antibody (CAB2526)

SKU:
CAB2526
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-TNK2 Antibody CAB2526
  • Cell Biology Antibodies 8 Anti-TNK2 Antibody CAB2526
€139 - €419
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Description

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Antibody Name:Anti-TNK2 Antibody
Antibody SKU:CAB2526
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-385 of human TNK2 (NP_001010938.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:SW620, HT-29, MCF7, Raji
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-385 of human TNK2 (NP_001010938.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TGWL LELL SEVQ LQQY FLRL RDDL NVTR LSHF EYVK NEDL EKIG MGRP GQRR LWEA VKRR KALC KRKS WMSK VFSG KRLE AEFP PHHS QSTF RKTS PAPG GPAG EGPL QSLT CLIG EKDL RLLE KLGD GSFG VVRR GEWD APSG KTVS VAVK CLKP DVLS QPEA MDDF IREV NAMH SLDH RNLI RLYG VVLT PPMK MVTE LAPL GSLL DRLR KHQG HFLL GTLS RYAV QVAE GMGY LESK RFIH RDLA ARNL LLAT RDLV KIGD FGLM RALP QNDD HYVM QEHR KVPF AWCA PESL KTRT FSHA SDTW MFGV TLWE
Gene ID:10188
Uniprot:Q07912
Cellular Location:Cell junction, Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endosome, Membrane, Nucleus, Peripheral membrane protein, adherens junction, clathrin-coated pit, clathrin-coated vesicle
Calculated MW:60kDa/114kDa/119kDa
Observed MW:115kDa
Synonyms:TNK2, ACK, ACK-1, ACK1, p21cdc42Hs
Background:This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
UniProt Protein Function:Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential. Two differentially spliced isoforms have been described.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.1; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: clathrin-coated vesicle; coated pit; cytoplasm; endosome; extrinsic to internal side of plasma membrane; membrane; nucleus; plasma membrane

Molecular Function:epidermal growth factor receptor binding; GTPase inhibitor activity; identical protein binding; protein binding; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; WW domain binding

Biological Process: cell differentiation; cell migration; cell surface receptor linked signal transduction; innate immune response; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell proliferation; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

NCBI Summary:This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q07912
NCBI GenInfo Identifier:229462980
NCBI Gene ID:10188
NCBI Accession:Q07912.3
UniProt Secondary Accession:Q07912,Q6ZMQ0, Q8N6U7, Q96H59,
UniProt Related Accession:Q07912
Molecular Weight:119,349 Da
NCBI Full Name:Activated CDC42 kinase 1
NCBI Synonym Full Names:tyrosine kinase non receptor 2
NCBI Official Symbol:TNK2  
NCBI Official Synonym Symbols:ACK; ACK1; ACK-1; p21cdc42Hs  
NCBI Protein Information:activated CDC42 kinase 1
UniProt Protein Name:Activated CDC42 kinase 1
UniProt Synonym Protein Names:Tyrosine kinase non-receptor protein 2
Protein Family:Activated CDC42 kinase
UniProt Gene Name:TNK2  
UniProt Entry Name:ACK1_HUMAN
Anti-TNK2 Antibody (CAB2526)Western blot analysis of extracts of various cell lines, using TNK2 antibody (CAB2526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-TNK2 Antibody (CAB2526)Immunofluorescence analysis of U2OS cells using TNK2 antibody (CAB2526). Blue: DAPI for nuclear staining.

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