The TNIP1 Polyclonal Antibody (PAC020730) is a crucial tool for researchers studying TNIP1, a protein involved in immune regulation and inflammatory pathways. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.TNIP1, also known as TNFAIP3 interacting protein 1, is a key player in regulating inflammatory responses and immune signaling pathways. By binding to TNIP1, this antibody allows for the detection and analysis of TNIP1 expression in a variety of cell types, making it an essential tool for studies related to immunology, inflammation, and autoimmune diseases.
The importance of TNIP1 in immune system modulation highlights its potential as a target for therapeutic interventions in diseases such as autoimmune disorders, chronic inflammatory conditions, and cancer. By understanding the role of TNIP1 in these conditions, researchers can develop new strategies for modulating immune responses and controlling inflammation.
Gel: 8%SDS-PAGE,Lysate: 40 µg,Lane 1-2: Mouse muscle tissue, Hela cells,Primary antibody: PACO20730(TNIP1 Antibody) at dilution 1/400 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 20 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20730(TNIP1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene.
Synonyms:
TNFAIP3 interacting protein 1
UniProt Protein Function:
TNIP1: Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal; however, binding to A20/TNFAIP3 seems not to be required for this function. Inhibits NF-kappa-B activation by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcricption. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflamation; this function is mediated by association with SELPLG and dependent on phosohorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection. Interacts with TNFAIP3 and IKBKG; facilitates TNFAIP3- mediated de-ubiquitination of NEMO/IKBKG. Interacts with HIV-1 matrix protein. Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IKBKG (polyubiquitinated). Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex. Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transcription regulation
Chromosomal Location of Human Ortholog: 5q32-q33.1
Molecular Function:protein binding; ubiquitin-specific protease activity; mitogen-activated protein kinase binding
Biological Process: MyD88-dependent toll-like receptor signaling pathway; leukocyte adhesion; negative regulation of viral genome replication; glycoprotein biosynthetic process; translation; defense response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; positive regulation of inflammatory response
NCBI Summary:
This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
