The TNFSF11 Polyclonal Antibody (CAB2550) is a versatile tool for researchers studying TNFSF11, a cytokine protein involved in bone metabolism and immune regulation. This antibody, produced using rabbit immunization, is specifically reactive with human samples and has been validated for use in Western blot applications. TNFSF11, also known as RANKL, is a key player in the regulation of osteoclast differentiation and activation, making it important for studies in bone biology and osteoporosis research. Additionally, TNFSF11 is involved in the immune response, particularly in the activation of T cells and dendritic cells.
The TNFSF11 Polyclonal Antibody binds specifically to TNFSF11 protein, enabling precise detection and analysis in various cell types. Its high specificity and sensitivity make it an ideal tool for researchers investigating the role of TNFSF11 in bone health, immune function, and diseases such as osteoporosis and rheumatoid arthritis. By understanding the mechanisms underlying TNFSF11 activity, researchers can develop targeted therapies for bone-related disorders and autoimmune conditions, ultimately leading to improved treatments for patients.
Product Name:
RANKL Rabbit Polyclonal Antibody
SKU:
CAB2550
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 210-309 of human RANKL (NP_003692.1).
Cell membrane, Cytoplasm, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein.
Calculated MW:
35kDa
Observed MW:
31kDa/45kDa
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
Purification Method:
Affinity purification
Gene ID:
8600
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RANKL antibody (CAB2550) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
