The TNFSF11 Polyclonal Antibody (CAB16326) is a powerful tool for researchers investigating TNFSF11, also known as RANKL, a key regulator of bone metabolism and immune response. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting TNFSF11 in human samples, making it a valuable asset in studies involving osteoporosis, bone remodeling, and immune cell activation.TNFSF11 is essential for the differentiation and activation of osteoclasts, the cells responsible for bone resorption. Dysregulation of TNFSF11 signaling has been implicated in various bone disorders, making it a promising therapeutic target for treating conditions like osteoporosis and bone metastases.
Additionally, TNFSF11 is involved in immune regulation, contributing to lymph node development and T cell activation.By utilizing the TNFSF11 Polyclonal Antibody, researchers can investigate the role of TNFSF11 in various physiological and pathological processes, shedding light on its therapeutic potential in bone-related diseases and immune disorders. Its reliability in Western blot applications ensures accurate and reproducible results, facilitating insightful research into the complex functions of TNFSF11 in health and disease.
Product Name:
RANKL Rabbit Polyclonal Antibody
SKU:
CAB16326
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human RANKL (NP_003692.1).
Cell membrane, Cytoplasm, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein.
Calculated MW:
35kDa
Observed MW:
35kDa
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
Purification Method:
Affinity purification
Gene ID:
8600
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RANKL antibody (CAB16326) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.
