The TNFAIP3 Polyclonal Antibody (CAB2127) is a specialized research tool designed for investigating TNFAIP3, a key regulator of inflammatory signaling pathways. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting.TNFAIP3, also known as A20, is a crucial anti-inflammatory protein that plays a vital role in dampening immune responses and maintaining immune homeostasis. Dysregulation of TNFAIP3 has been implicated in various inflammatory diseases, making it an important target for research in immunology and inflammation-related disorders.
By specifically binding to TNFAIP3, this antibody enables precise detection and analysis of the protein in different cell types, allowing for a deeper understanding of its function in various pathological conditions. Research utilizing the TNFAIP3 Polyclonal Antibody CAB2127 can contribute valuable insights into the mechanisms underlying inflammatory diseases and provide potential avenues for therapeutic intervention.
Product Name:
TNFAIP3 Rabbit Polyclonal Antibody
SKU:
CAB2127
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 600-738 of human TNFAIP3 (NP_006281.1).
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7128
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CAB2127) at 1:500 dilution.THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.
