The TNF Polyclonal Antibody is a valuable tool for research involving tumor necrosis factor (TNF), a key regulator of inflammation and immune response. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blotting applications. By targeting the TNF protein, this antibody allows for the detection and analysis of TNF levels in various cell types, making it an essential component for studies in immunology, inflammatory diseases, and cancer research.TNF is a cytokine that plays a crucial role in the regulation of immune and inflammatory responses.
Dysregulation of TNF signaling is associated with various diseases, including rheumatoid arthritis, inflammatory bowel disease, and certain types of cancer. By studying the activity of TNF using this antibody, researchers can gain a better understanding of its role in disease pathology and potentially identify new therapeutic targets for intervention.Overall, the TNF Polyclonal Antibody is a reliable tool for investigating the function of TNF in different disease contexts, contributing to the advancement of knowledge in immunology and the development of targeted therapies for a range of conditions.
Product Name:
TNF-α Polyclonal Antibody
SKU:
CAB21769
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-235 of mouse TNF-α (NP_038721.1).
cell surface, cytoplasm, external side of plasma membrane, extracellular region, extracellular space, phagocytic cup, plasma membrane, recycling endosome
Calculated MW:
26kDa
Observed MW:
Refer to figures
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
21926
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using TNF-α antibody (CAB21769) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
