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TNF-α Monoclonal Antibody (CAB22227)

SKU:
CAB22227
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:TNF-α Monoclonal Antibody
SKU:CAB22227
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-α (NP_000585.2).
Sequence:VRSS SRTP SDKP VAHV VANP QAEG QLQW LNRR ANAL LANG VELR DNQL VVPS EGLY LIYS QVLF KGQG CPST HVLL THTI SRIA VSYQ TKVN LLSA IKSP CQRE TPEG AEAK PWYE PIYL GGVF QLEK GDRL SAEI NRPD YLDF AESG QVYF GIIA L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:10000 - 1:40000
Synonyms:DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNF-α
Positive Sample:THP-1+LPS
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Membrane, Secreted.
Calculated MW:26kDa
Observed MW:18kDa/25kDa

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Purification Method:Affinity purification
Gene ID:7124
Clone Number:ARC52531
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using TNF-α Rabbit mAb (CAB22227) at1:34000 dilution.THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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