The TNF Monoclonal Antibody (CAB22227) is a highly specific and sensitive tool for researchers studying tumor necrosis factor (TNF), a key cytokine involved in inflammation and immune response. This antibody, produced through advanced monoclonal technology, recognizes and binds specifically to TNF in human samples, making it an essential reagent for various immunological and cancer-related studies.TNF is a critical regulator of inflammatory processes and has been implicated in a wide range of diseases, including autoimmune disorders, infectious diseases, and cancer. By targeting TNF with this monoclonal antibody, researchers can gain important insights into the role of TNF in disease pathogenesis and potentially identify new therapeutic strategies for TNF-associated conditions.
Whether used for Western blot analysis, immunofluorescence, or flow cytometry, the TNF Monoclonal Antibody (CAB22227) offers reliable and reproducible results, allowing researchers to accurately detect and quantify TNF expression in different experimental settings. Its high specificity and sensitivity make it a valuable tool for investigating TNF biology and developing targeted therapies for TNF-related disorders.
Product Name:
TNF-α Monoclonal Antibody
SKU:
CAB22227
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-α (NP_000585.2).
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Purification Method:
Affinity purification
Gene ID:
7124
Clone Number:
ARC52531
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TNF-α Rabbit mAb (CAB22227) at1:34000 dilution.THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
