The TNF-alpha Polyclonal Antibody (CAB11534) is a valuable tool for researchers studying tumor necrosis factor alpha (TNF-alpha), a key cytokine involved in inflammation and immune response regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in applications such as Western blotting.TNF-alpha is a pro-inflammatory cytokine that plays a central role in various disease processes, including autoimmune disorders, inflammatory conditions, and cancer. By targeting TNF-alpha, researchers can gain insight into the mechanisms underlying these diseases and potentially develop novel therapeutic strategies.
The TNF-alpha Polyclonal Antibody binds specifically to TNF-alpha, allowing for precise detection and analysis in a variety of cell types. Its high reactivity and specificity make it an essential tool for studies in immunology, oncology, and inflammation research. By understanding the role of TNF-alpha in disease processes, researchers can advance our knowledge of immune regulation and develop targeted therapies for a range of conditions.
Product Name:
TNF-α Rabbit Polyclonal Antibody
SKU:
CAB11534
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 134-233 of human TNF-α (NP_000585.2).
Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein.
Calculated MW:
26kDa
Observed MW:
18kDa/25kDa
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Purification Method:
Affinity purification
Gene ID:
7124
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using TNF-α Rabbit pAb (CAB11534) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
