The TMEM64 Polyclonal Antibody (PAC046286) is a valuable tool for researchers studying TMEM64, a transmembrane protein with potential relevance in various cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting TMEM64 in human samples, making it ideal for use in experiments such as Western blotting.TMEM64 has been identified as a protein involved in ion transport and cellular homeostasis, suggesting its importance in cellular function and potentially impacting diseases such as cancer and neurodegenerative disorders.
The ability to accurately detect and analyze TMEM64 using this antibody opens up opportunities for further exploration of its role in these pathologies, offering insights that may contribute to the development of targeted therapies.Given the emerging significance of TMEM64 in cellular biology and disease mechanisms, the TMEM64 Polyclonal Antibody serves as a valuable tool for researchers seeking to unravel the complexities of this protein and its implications in various physiological and pathological conditions.
Western blot. All lanes: TMEM64 antibody at 8µg/ml + Mouse brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 40, 13, 15, 34 kDa. Observed band size: 40 kDa..
Immunohistochemistry of paraffin-embedded human lung tissue using PACO46286 at dilution of 1:100.
Background:
Positively regulates TNFSF11-induced osteoclast differentiation. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with SERCA2 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation. Negatively regulates osteoblast differentiation and positively regulates adipocyte differentiation via modulation of the canonical Wnt signaling pathway. Mediates the switch in lineage commitment to osteogenesis rather than to adipogenesis in mesenchymal stem cells by negatively regulating the expression, activity and nuclear localization of CTNNB1.
Synonyms:
Transmembrane protein 64, TMEM64
UniProt Protein Function:
TMEM64: Belongs to the TVP38/TMEM64 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 8q21.3Biological Process: negative regulation of osteoblast differentiation; positive regulation of fat cell differentiation
