The TMEM231 Polyclonal Antibody (PACO60328) is a valuable tool for researchers studying TMEM231, a protein involved in various cellular processes, including ciliary function and development. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to TMEM231, researchers can detect and analyze this protein in different cell types, making it ideal for investigations in cell biology, developmental biology, and ciliopathies.TMEM231 is a transmembrane protein localized in the primary cilium, where it plays a crucial role in ciliary development and function.
Dysregulation of TMEM231 has been linked to ciliopathies, a group of genetic disorders characterized by defects in cilia structure and function. Studying the function of TMEM231 can provide insights into the mechanisms underlying ciliary-related diseases, with potential implications for therapeutic interventions and treatments. The TMEM231 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the roles of TMEM231 in health and disease.
Antibody Name:
TMEM231 Antibody (PACO60328)
Antibody SKU:
PACO60328
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Transmembrane protein 231 protein (161-261AA)
Immunofluorescence staining of MCF-7 cells with PACO60328 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling (By similarity).
Synonyms:
Transmembrane protein 231, TMEM231
UniProt Protein Function:
TMEM231: Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling. Belongs to the TMEM231 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 16q23.1Cellular Component: integral to membraneBiological Process: smoothened signaling pathway; cilium biogenesisDisease: Joubert Syndrome 1; Meckel Syndrome, Type 11; Joubert Syndrome 20
UniProt Protein Details:
NCBI Summary:
This gene encodes a transmembrane protein, which is a component of the B9 complex involved in the formation of the diffusion barrier between the cilia and plasma membrane. Mutations in this gene cause Joubert syndrome (JBTS). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]
