The TMEM127 Antibody (PAC060845) is a vital tool for researchers studying the TMEM127 protein, a key player in cellular homeostasis and tumor suppression. This polyclonal antibody, developed using advanced technology, is highly specific to human samples and is suitable for use in a variety of applications, including Western blot and immunohistochemistry.TMEM127 is a transmembrane protein known for its role in maintaining cellular balance and inhibiting tumor growth. By targeting TMEM127 with this antibody, researchers can analyze its expression levels and localization within cells, providing valuable insights into its function in various biological processes.
The significance of TMEM127 in cancer research and cellular homeostasis makes it a promising target for further investigation. The TMEM127 Antibody (PAC060845) offers researchers a reliable tool to explore the mechanisms underlying TMEM127's tumor-suppressive properties and its potential as a therapeutic target in cancer treatment.
IHC image of PACO60845 diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO60845 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.
Synonyms:
Transmembrane protein 127, TMEM127
UniProt Protein Function:
Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.
UniProt Protein Details:
NCBI Summary:
This gene encodes a transmembrane protein with 3 predicted transmembrane domains. The protein is associated with a subpopulation of vesicular organelles corresponding to early endosomal structures, with the Golgi, and with lysosomes, and may participate in protein trafficking between these structures. Mutations in this gene and several other genes cause pheochromocytomas. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2010]
