The TMC5 Polyclonal Antibody (CAB16145) offered by AssayGenie is a valuable tool for researchers studying TMC5, a protein associated with the transmembrane channel-like (TMC) family involved in ion channel functions and cell signaling processes. This antibody, produced in rabbits, exhibits high reactivity with samples of human origin and is optimized for Western blot applications.TMC5 is known to play a role in various cellular processes, including calcium signaling and sensory transduction mechanisms. Its expression in different tissues and cell types suggests a diverse functional significance, making it a key target for investigation in molecular biology and cell physiology studies.
By utilizing the TMC5 Polyclonal Antibody, researchers can effectively detect and analyze TMC5 protein levels in a range of experimental settings, enabling a deeper understanding of its biological functions and potential implications in diseases related to ion channel dysregulation and cellular signaling pathways. This antibody is a valuable resource for furthering research in areas such as neuroscience, physiology, and drug development.
Product Name:
TMC5 Rabbit Polyclonal Antibody
SKU:
CAB16145
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 676-760 of human TMC5 (NP_079056.2).
Sequence:
YWQI TEGR KIMI RLLH EQII NEGK DKMF LIEK LIKL QDME KKAN PSSL VLER REVE QQGF LHLG EHDG SLDL RSRR SVQE GNPR A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
TMC5
Positive Sample:
Mouse testis,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Membrane, Multi-pass membrane protein
Calculated MW:
115kDa
Observed MW:
114kDa
Predicted to enable mechanosensitive ion channel activity. Predicted to be involved in ion transmembrane transport. Located in extracellular exosome.
Purification Method:
Affinity purification
Gene ID:
79838
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TMC5 Rabbit pAb (CAB16145) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
