The TLR9 Polyclonal Antibody (CAB14642) is a valuable tool for researchers studying Toll-like receptor 9 (TLR9), a key player in the innate immune system. The antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications. By targeting TLR9, this antibody enables the detection and analysis of this important protein in a variety of cell types, making it an essential component for studies in immunology and infectious disease research.
TLR9 is a pattern recognition receptor that plays a crucial role in detecting microbial DNA and initiating immune responses against pathogens. Its involvement in the activation of the immune system makes it a potential target for therapeutic interventions in various diseases, including infectious diseases, autoimmune disorders, and cancer. By understanding the function and regulation of TLR9, researchers can develop novel strategies for modulating immune responses and combating disease.
Product Name:
TLR9 Rabbit Polyclonal Antibody
SKU:
CAB14642
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 414-543 of human TLR9 (NP_059138.1).
Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Lysosome, Single-pass type I membrane protein, phagosome.
Calculated MW:
116kDa
Observed MW:
135kDa
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Purification Method:
Affinity purification
Gene ID:
54106
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using TLR9 Rabbit pAb (CAB14642) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
