The TLR4 Polyclonal Antibody (CAB5258) is a key tool for researchers studying Toll-like receptor 4 (TLR4), a protein involved in the immune response to bacterial lipopolysaccharides. This antibody is produced in rabbits and is highly specific for TLR4 in human samples, making it a reliable choice for Western blot applications. By binding to TLR4, this antibody allows for the detection and analysis of TLR4 expression in various cell types, making it ideal for studies in immunology, infectious diseases, and inflammation research.
TLR4 plays a crucial role in the recognition of pathogens and activation of the innate immune response. Dysregulation of TLR4 signaling has been linked to inflammatory diseases, sepsis, and autoimmune disorders. Understanding the function of TLR4 is essential for developing targeted therapies that modulate the immune response in these conditions. The TLR4 Polyclonal Antibody (CAB5258) offers researchers a valuable tool for investigating the role of TLR4 in health and disease.
Product Name:
TLR4 Rabbit Polyclonal Antibody
SKU:
CAB5258
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-228 of human TLR4 (NP_612564.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
96kDa
Observed MW:
100-135kDa
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TLR4 Rabbit pAb (CAB5258) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
