The TLR4 Polyclonal Antibody (CAB11226) is a valuable tool for research involving Toll-like Receptor 4 (TLR4), a critical component of the innate immune system responsible for recognizing bacterial lipopolysaccharides. The antibody, produced in rabbits, shows high reactivity with human TLR4 and is validated for use in Western blot and immunohistochemistry applications. By specifically binding to TLR4, this antibody enables precise detection and analysis of TLR4 expression in various cell types, making it indispensable for studies in immunology and infectious disease research.
TLR4 is essential for initiating inflammatory responses to bacterial infections and is implicated in the pathogenesis of various diseases, including sepsis, inflammatory disorders, and autoimmune conditions. Understanding the role of TLR4 in immune activation and inflammation is crucial for developing targeted therapies and vaccines against infectious agents and inflammatory diseases. The TLR4 Polyclonal Antibody provides researchers with a reliable tool to investigate the function and regulation of TLR4 in health and disease contexts.
Product Name:
[KO Validated] TLR4 Rabbit Polyclonal Antibody
SKU:
CAB11226
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-228 of human TLR4 (NP_612564.1).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TOLL; CD284; TLR-4; ARMD10; TLR4
Positive Sample:
RAW264.7
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
96kDa
Observed MW:
100-135kDa
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TLR4 Rabbit pAb (CAB11226) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
