The TLR4 Polyclonal Antibody (CAB0007) is a vital tool for researchers investigating Toll-like receptor 4 (TLR4), a key player in the innate immune response. This antibody, produced in rabbits, exhibits high specificity for human samples and has been rigorously validated for use in Western blotting applications. By targeting the TLR4 protein, this antibody enables precise detection and analysis of TLR4 expression in various cell types, making it an invaluable resource for studies in immunology and infectious disease research.
TLR4 is a crucial component of the immune system, responsible for recognizing pathogen-associated molecular patterns and initiating inflammatory responses. Dysregulation of TLR4 signaling has been implicated in various diseases, including sepsis, autoimmune disorders, and chronic inflammatory conditions. By studying the role of TLR4 using this antibody, researchers can gain insights into how the immune system responds to pathogens and develop novel therapeutic strategies targeting TLR4 signaling pathways.
Product Name:
TLR4 Rabbit Polyclonal Antibody
SKU:
CAB0007
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-228 of human TLR4 (NP_612564.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
96kDa
Observed MW:
100-135kDa
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TLR4 Rabbit pAb (CAB0007) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
