The TLK1 Polyclonal Antibody (PACO05832) is a valuable tool for researchers studying the TLK1 protein, which plays a crucial role in cell cycle regulation and DNA repair processes. This antibody, produced in rabbits, has high specificity for TLK1 and has been validated for use in Western blotting and immunofluorescence applications.TLK1 is a serine/threonine kinase that is involved in the maintenance of genomic stability and the response to DNA damage. Dysregulation of TLK1 has been linked to various diseases, including cancer and developmental disorders.
By using the TLK1 Polyclonal Antibody, researchers can effectively detect and analyze the TLK1 protein in different cell types, providing valuable insights into its function and potential therapeutic targets.Overall, the TLK1 Polyclonal Antibody is an essential tool for researchers interested in unraveling the complex mechanisms of cell cycle regulation and DNA repair, with implications for understanding and treating a wide range of diseases.
Antibody Name:
TLK1 Antibody (PACO05832)
Antibody SKU:
PACO05832
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:5000, IF:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from human TLK1 around the non-phosphorylation site of S764.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
TLK1 iso1: a protein kinase of the TLK family. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation, possibly by CHK1. Three splice variant isoforms have been described.Protein type: Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; TLK familyChromosomal Location of Human Ortholog: 2q31.1Cellular Component: nucleusMolecular Function: protein serine/threonine kinase activity; ATP bindingBiological Process: intracellular protein transport; regulation of chromatin assembly or disassembly; chromatin modification; cell cycle; response to DNA damage stimulus; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
