The TJP1 Polyclonal Antibody (CAB11417) is a valuable tool for researchers studying TJP1, a key protein involved in the regulation of cell-cell adhesion and barrier function in epithelial and endothelial cells. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications.TJP1, also known as ZO-1, is a crucial component of tight junctions, which play a critical role in maintaining the integrity of epithelial and endothelial barriers. Dysregulation of TJP1 has been implicated in various diseases, including cancer, inflammatory bowel disease, and barrier dysfunction disorders.
By targeting TJP1 with this polyclonal antibody, researchers can investigate its expression levels and localization in different cell types, thereby advancing our understanding of its role in disease pathology.Overall, the TJP1 Polyclonal Antibody (CAB11417) is a reliable tool for studies in cell biology, cancer research, and epithelial barrier function, providing valuable insights into the molecular mechanisms underlying TJP1-mediated cellular processes.
Product Name:
ZO-1 Rabbit Polyclonal Antibody
SKU:
CAB11417
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-273 of human ZO-1 (NP_003248.3).
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
7082
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZO-1 Rabbit pAb (CAB11417) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
