The TIE1 Polyclonal Antibody (CAB3334) is a key tool for researchers studying TIE1, a tyrosine kinase receptor involved in angiogenesis and vascular development. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blotting. By binding to the TIE1 protein, this antibody enables the detection and analysis of TIE1 expression in different cell types, making it a valuable asset for studies in vascular biology and cancer research.
TIE1 plays a crucial role in regulating blood vessel formation and maintaining vascular integrity, making it a promising target for therapy development in diseases related to angiogenesis, such as cancer and cardiovascular disorders. Understanding the function and regulation of TIE1 is essential for advancing our knowledge of vascular biology and potentially identifying new treatment strategies for these conditions.
Product Name:
TIE1 Rabbit Polyclonal Antibody
SKU:
CAB3334
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human TIE1 (NP_005415.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
125kDa
Observed MW:
135/140kDa
This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
7075
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TIE1 Rabbit pAb (CAB3334) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
