The Thymidylate Kinase Polyclonal Antibody (CAB6370) is a valuable tool for researchers studying thymidylate kinase, an enzyme involved in DNA synthesis and repair. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the thymidylate kinase protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an excellent choice for studies in molecular biology and cancer research.
Thymidylate kinase is a key enzyme in the pyrimidine nucleotide biosynthesis pathway, essential for DNA replication and cell proliferation. Its role in maintaining genomic stability and promoting cell growth makes it a promising target for therapeutic interventions in diseases like cancer. By investigating the function and regulation of thymidylate kinase, researchers can gain valuable insights into pathways that drive tumorigenesis and potential strategies for targeted treatment.
Product Name:
DTYMK Rabbit Polyclonal Antibody
SKU:
CAB6370
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human DTYMK (NP_036277.2).
22Rv1,HeLa,HepG2,Raji,MCF7,Mouse bone marrow,Mouse spleen,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, mitochondrion, nucleus
Calculated MW:
24kDa
Observed MW:
24kDa
Enables thymidylate kinase activity. Predicted to be involved in dTDP biosynthetic process; dTTP biosynthetic process; and dUDP biosynthetic process. Predicted to act upstream of or within cellular response to growth factor stimulus and nucleotide biosynthetic process. Predicted to be located in mitochondrial intermembrane space and mitochondrial matrix. Predicted to be active in cytosol; mitochondrion; and nucleus.
Purification Method:
Affinity purification
Gene ID:
1841
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DTYMK antibody (CAB6370) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
