The Thrombopoietin Polyclonal Antibody (CAB1825) is a valuable tool for researchers studying thrombopoietin, a protein involved in the regulation of platelet production. This antibody, developed in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to thrombopoietin, the antibody enables accurate detection and analysis of this important protein in various cell types.Thrombopoietin plays a crucial role in hematopoiesis, particularly in the regulation of megakaryocyte development and platelet formation.
Dysregulation of thrombopoietin levels can lead to disorders such as thrombocytopenia or thrombocythemia, highlighting the significance of studying this protein in both normal and pathological conditions. The Thrombopoietin Polyclonal Antibody provides researchers with a reliable tool for investigating the role of thrombopoietin in hematological disorders and developing potential therapeutic interventions.
Product Name:
THPO Rabbit Polyclonal Antibody
SKU:
CAB1825
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1).
Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Purification Method:
Affinity purification
Gene ID:
7066
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using THPO antibody (CAB1825) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
