The THOP1 Polyclonal Antibody (CAB8756) is a valuable tool for researchers studying THOP1, a metalloprotease enzyme involved in the degradation of bioactive peptides. This antibody, produced through immunization of rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to THOP1 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in protease biology and peptide metabolism.THOP1, also known as thimet oligopeptidase, plays a crucial role in the regulation of peptide signaling pathways and neuropeptide metabolism.
Its importance in peptide degradation and processing makes it a key player in various physiological processes, including neurotransmission, immune response modulation, and cardiovascular function. Research on THOP1 is essential for unraveling its implications in diseases like Alzheimer's, hypertension, and inflammatory disorders, paving the way for the development of targeted therapeutic interventions.
Product Name:
THOP1 Rabbit Polyclonal Antibody
SKU:
CAB8756
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human THOP1 (NP_003240.1).
The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides.
Purification Method:
Affinity purification
Gene ID:
7064
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using THOP1 antibody (CAB8756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
