The THOP1 Antibody (CAB8756) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to THOP1 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in protease biology and peptide metabolism.THOP1, also known as thimet oligopeptidase, plays a crucial role in the regulation of peptide signaling pathways and neuropeptide metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
THOP1 Antibody
SKU:
CAB8756
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TOP, MP78, EP24.15, MEPD_HUMAN, THOP1
Positive Sample:
Mouse skeletal muscle, Mouse heart, Mouse lung, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides.
Purification Method
Affinity purification
Gene ID
7064
RRID
AB_2772596
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using THOP1 Rabbit pAb (CAB8756) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
