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THEMIS Antibody (PACO57384)

SKU:
PACO57384
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • THEMIS Antibody PACO57384
  • THEMIS Antibody PACO57384
  • THEMIS Antibody PACO57384
€359

Description

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THEMIS Antibody (PACO57384)

The Themis Antibody (PAC057384) is a highly specific and sensitive tool for studying the Themis protein, an important regulator of T cell development and function. This polyclonal antibody is produced in rabbits and is optimized for detecting Themis in human samples, making it an ideal choice for immunology and cancer research studies.Themis is a key signaling molecule that plays a critical role in T cell receptor signaling and immune system function. Dysregulation of Themis has been implicated in various diseases, including autoimmune disorders and cancer.

By using the Themis Antibody, researchers can better understand the mechanisms by which Themis influences immune responses, potentially leading to the development of targeted therapies for these conditions.Validated for use in Western blot applications, the Themis Antibody allows for precise detection and analysis of Themis expression in various cell types. With its high reactivity and specificity, this antibody is a valuable tool for researchers looking to delve deeper into the immune system and its impact on human health.

Antibody Name:THEMIS Antibody (PACO57384)
Antibody SKU:PACO57384
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:Human, Rat
Immunogen:Recombinant Human Protein THEMIS protein (547-641AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate. All lanes: THEMIS antibody at 7.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 74, 70, 63, 78 kDa. Observed band size: 74, 70 kDa.
Product ImageIHC image of PACO57384 diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageImmunofluorescence staining of HepG2 cells with PACO57384 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Synonyms:Protein THEMIS (Thymocyte-expressed molecule involved in selection), THEMIS, C6orf190 C6orf207
UniProt Protein Function:THEMIS: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. Interacts with PLCG1, ITK and GRB2. Belongs to the themis family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiationChromosomal Location of Human Ortholog: 6q22.33Cellular Component: cytoplasm; intercellular junction; nucleus; signalosomeMolecular Function: protein bindingBiological Process: adaptive immune response; negative T cell selection; positive T cell selection; T cell receptor signaling pathway
UniProt Protein Details:
NCBI Summary:This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Code:Q8N1K5
NCBI GenInfo Identifier:150421530
NCBI Gene ID:387357
NCBI Accession:Q8N1K5.3
UniProt Secondary Accession:Q8N1K5,Q5T3C4, Q5T3C5, Q6MZT7, A1L4F0, A8K7N1, B3KT31 B3KW32, B3KY07, F5H1J9
UniProt Related Accession:Q8N1K5
Molecular Weight:77,515 Da
NCBI Full Name:Protein THEMIS
NCBI Synonym Full Names:thymocyte selection associated
NCBI Official Symbol:THEMIS  
NCBI Official Synonym Symbols:GASP; SPOT; TSEPA; C6orf190; C6orf207  
NCBI Protein Information:protein THEMIS
UniProt Protein Name:Protein THEMIS
UniProt Synonym Protein Names:Thymocyte-expressed molecule involved in selection
Protein Family:Protein
UniProt Gene Name:THEMIS  
UniProt Entry Name:THMS1_HUMAN
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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