THEMIS Antibody (PACO57384)
- SKU:
- PACO57384
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | THEMIS Antibody (PACO57384) |
| Antibody SKU: | PACO57384 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human Protein THEMIS protein (547-641AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate. All lanes: THEMIS antibody at 7.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 74, 70, 63, 78 kDa. Observed band size: 74, 70 kDa. |
 | IHC image of PACO57384 diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO57384 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. |
| Synonyms: | Protein THEMIS (Thymocyte-expressed molecule involved in selection), THEMIS, C6orf190 C6orf207 |
| UniProt Protein Function: | THEMIS: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. Interacts with PLCG1, ITK and GRB2. Belongs to the themis family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiationChromosomal Location of Human Ortholog: 6q22.33Cellular Component: cytoplasm; intercellular junction; nucleus; signalosomeMolecular Function: protein bindingBiological Process: adaptive immune response; negative T cell selection; positive T cell selection; T cell receptor signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] |
| UniProt Code: | Q8N1K5 |
| NCBI GenInfo Identifier: | 150421530 |
| NCBI Gene ID: | 387357 |
| NCBI Accession: | Q8N1K5.3 |
| UniProt Secondary Accession: | Q8N1K5,Q5T3C4, Q5T3C5, Q6MZT7, A1L4F0, A8K7N1, B3KT31 B3KW32, B3KY07, F5H1J9 |
| UniProt Related Accession: | Q8N1K5 |
| Molecular Weight: | 77,515 Da |
| NCBI Full Name: | Protein THEMIS |
| NCBI Synonym Full Names: | thymocyte selection associated |
| NCBI Official Symbol: | THEMISÂ Â |
| NCBI Official Synonym Symbols: | GASP; SPOT; TSEPA; C6orf190; C6orf207Â Â |
| NCBI Protein Information: | protein THEMIS |
| UniProt Protein Name: | Protein THEMIS |
| UniProt Synonym Protein Names: | Thymocyte-expressed molecule involved in selection |
| Protein Family: | Protein |
| UniProt Gene Name: | THEMISÂ Â |
| UniProt Entry Name: | THMS1_HUMAN |