Anti-TGM3 Antibody (CAB5856)
- SKU:
- CAB5856
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-TGM3 Antibody (CAB5856)
The TGM3 Polyclonal Antibody (CAB5856) is a valuable tool for researchers studying transglutaminase 3 (TGM3), an enzyme involved in various biological processes including cell differentiation and skin development. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.TGM3 is known for its role in cross-linking proteins in the skin, contributing to the formation of the epidermal barrier. Dysregulation of TGM3 expression has been associated with conditions such as psoriasis and atopic dermatitis, highlighting its importance in skin biology and disease pathology.
By targeting TGM3 with this antibody, researchers can investigate its expression and activity in different cell types and tissues, providing insights into its function and potential therapeutic applications in dermatology and related fields.Overall, the TGM3 Polyclonal Antibody offers a valuable tool for studies aimed at understanding the role of TGM3 in skin physiology and pathology, paving the way for the development of targeted therapies for skin disorders and related conditions.
| Antibody Name: | Anti-TGM3 Antibody |
| Antibody SKU: | CAB5856 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, A-549, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL GVQS INWQ TAFN RQAH HTDK FSSQ ELIL RRGQ NFQV LMIM NKGL GSNE RLEF IVST GPYP SESA MTKA VFPL SNGS SGGW SAVL QASN GNTL TISI SSPA SAPI GRYT MALQ IFSQ GGIS SVKL GTFI LLFN PWLN VDSV FMGN HAER EEYV QEDA GIIF VGST NRIG MIGW NFGQ |
| Gene ID: | 7053 |
| Uniprot: | Q08188 |
| Cellular Location: | |
| Calculated MW: | 76kDa |
| Observed MW: | 72kDa |
| Synonyms: | TGM3, TGE, UHS2 |
| Background: | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. |
| UniProt Protein Function: | TGM3: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross- linking structural proteins to hardening the inner root sheath. Belongs to the transglutaminase superfamily. Transglutaminase family. |
| UniProt Protein Details: | Protein type:EC 2.3.2.13; Membrane protein, peripheral; Transferase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm Molecular Function:protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups; calcium ion binding; catalytic activity Biological Process: keratinocyte differentiation; cell envelope organization and biogenesis; hair follicle morphogenesis; keratinization; protein modification process; peptide cross-linking; protein tetramerization |
| NCBI Summary: | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q08188 |
| NCBI GenInfo Identifier: | 257051080 |
| NCBI Gene ID: | 7053 |
| NCBI Accession: | Q08188.4 |
| UniProt Secondary Accession: | Q08188,O95933, Q32ML9, Q32MM0, A8K5N6, B2RCR6, D3DVX1 |
| UniProt Related Accession: | Q08188 |
| Molecular Weight: | |
| NCBI Full Name: | Protein-glutamine gamma-glutamyltransferase E |
| NCBI Synonym Full Names: | transglutaminase 3 |
| NCBI Official Symbol: | TGM3Â Â |
| NCBI Official Synonym Symbols: | TGEÂ Â |
| NCBI Protein Information: | protein-glutamine gamma-glutamyltransferase E; TG(E); TGase E; TGase-3; transglutaminase E; transglutaminase-3; E polypeptide, protein-glutamine-gamma-glutamyltransferase |
| UniProt Protein Name: | Protein-glutamine gamma-glutamyltransferase E |
| UniProt Synonym Protein Names: | Transglutaminase E; TG(E); TGE; TGase E; Transglutaminase-3; TGase-3 |
| Protein Family: | Protein-glutamine gamma-glutamyltransferase |
| UniProt Gene Name: | TGM3Â Â |
| UniProt Entry Name: | TGM3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TGM3 antibody (CAB5856) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 50s. |
 | Immunofluorescence analysis of U2OS cells using TGM3 antibody (CAB5856). Blue: DAPI for nuclear staining. |
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