The TGIF1 Polyclonal Antibody (CAB16984) is a valuable tool for research involving TGIF1, a transcriptional repressor involved in regulating gene expression in various cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By targeting the TGIF1 protein, researchers can accurately detect and analyze its expression in different cell types, providing insights into its role in biological pathways.TGIF1, also known as TGF-beta-inducible early gene-1, is involved in modulating key signaling pathways, including those mediated by transforming growth factor beta (TGF-β).
Its functions in controlling gene transcription and cell proliferation make it a relevant target for studies in developmental biology, cancer research, and stem cell regulation. Investigating the activity and regulation of TGIF1 can provide valuable information for understanding disease mechanisms and identifying potential therapeutic targets.Overall, the TGIF1 Polyclonal Antibody (CAB16984) offers researchers a reliable tool for investigating the role of TGIF1 in cellular processes and disease pathways, contributing to advancements in biomedical research and drug discovery efforts.
Product Name:
TGIF1 Polyclonal Antibody
SKU:
CAB16984
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-340 of human TGIF1 (NP_733796.2).
The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.
Purification Method:
Affinity purification
Gene ID:
7050
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TGIF1 Rabbit pAb (CAB16984) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
