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TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit

SKU:
AEES00020
€499
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Description

Product Name: TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit
SKU: AEES00020
Synonyms: Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1
Size: 96T
Assay Time: 3.5 hours
Sample Volume: 100μL
Sensitivity: 0.10 ng/mL
Detection range: 0.16-10 ng/mL
Sample Size: Run up to 46 samples in duplicate and 30 samples in triplicate.
Kit component:
Component Quantity (24 Assays) Quantity (96 Assays) Storage
Micro ELISA Plate (Dismountable) 8 wells x 3 strips 8 wells x 12 strips -20°C, 12 months
Reference Standard 1 vial 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 60 µL 1 vial, 120 µL
Concentrated HRP Conjugate (100×) 1 vial, 60 µL 1 vial, 120 µL -20°C (shading light), 12 months
Activator reagent 1 (1M HCL) 1 vial, 5 mL 1 vial, 5 mL 2-8°C (protect from sunlight), 12 months
Activator reagent 2 (1.2M NaOH/0.5M HEPES) 1 vial, 5 mL 1 vial, 5 mL
Reference Standard & Sample Diluent 1 vial, 20 mL 1 vial, 20 mL 4°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 1 vial, 10 mL 4°C (shading light)
Stop Solution 1 vial, 10 mL 1 vial, 10 mL 4°C
Plate Sealer 5 pieces 5 pieces
Product Description 1 copy 1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 99-108 95-105 92-104
Average (%) 105 102 97
1:4 Range (%) 95-109 90-100 97-105
Average (%) 102 96 100
1:8 Range (%) 89-103 89-104 96-108
Average (%) 97 97 102
1:16 Range (%) 88-102 98-108 92-105
Average (%) 94 104 100
Recovery:
Sample Type Range (%) Average Recovery (%)
Serum (n=5) 94-110 102
EDTA plasma (n=5) 90-102 96
Cell culture media (n=5) 95-106 98
Precision:
Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20.0 20.0 20.0 20.0 20.0
Mean (ng/mL) 0.41 1.07 4.97 0.47 1.25 4.24
Standard deviation 0.02 0.05 0.22 0.03 0.06 0.2
C V (%) 4.88 4.67 4.43 6.38 4.8 4.72
Sample Type: Serum, Plasma and Other Biological fluids
Reproducibility: Both intra-CV and inter-CV are < 10%.
Application: Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Storage: 2-8℃

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