The TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit is expertly designed for the quantitative measurement of TGF-β1 levels in various biological samples.
TGF-β1 is a potent multi-functional cytokine implicated in numerous biological processes, such as cell proliferation, differentiation, and apoptosis. It holds a critical role in the immune system by regulating immune responses and is associated with several pathological conditions, including inflammatory diseases, autoimmune disorders, and fibrosis. Precise quantification of TGF-β1 is crucial for understanding its contribution to disease pathogenesis and for the development of targeted therapeutic interventions. Assay Genie's TGF-β1 ELISA Kit ensures optimal sensitivity and specificity, providing reliable and consistent results. Produced under strict quality control measures, this kit offers robust performance and simplicity of use, making it an outstanding choice for research and clinical applications alike. Rely on Assay Genie's TGF-β1 ELISA Kit for reliable and precise quantification of this vital biomarker in your investigations.
Run up to 46 samples in duplicate and 30 samples in triplicate.
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Activator reagent 1 (1M HCL)
1 vial, 5 mL
1 vial, 5 mL
2-8°C (protect from sunlight), 12 months
Activator reagent 2 (1.2M NaOH/0.5M HEPES)
1 vial, 5 mL
1 vial, 5 mL
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-108
95-105
92-104
Average (%)
105
102
97
1:4
Range (%)
95-109
90-100
97-105
Average (%)
102
96
100
1:8
Range (%)
89-103
89-104
96-108
Average (%)
97
97
102
1:16
Range (%)
88-102
98-108
92-105
Average (%)
94
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
95-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.41
1.07
4.97
0.47
1.25
4.24
Standard deviation
0.02
0.05
0.22
0.03
0.06
0.2
C V (%)
4.88
4.67
4.43
6.38
4.8
4.72
Sample Type:
Serum, Plasma and Other Biological fluids
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
