Anti-TFDP1 Antibody (CAB5422)

Antibody Name:	Anti-TFDP1 Antibody
Antibody SKU:	CAB5422
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human TFDP1 (NP_009042.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	LO2, Raji, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human TFDP1 (NP_009042.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKD AGLI EANG ELKV FIDQ NLSP GKGV VSLV AVHP STVN PLGK QLLP KTFG QSNV NIAQ QVVI GTPQ RPAA SNTL VVGS PHTP STHF ASQN QPSD SSPW SAGK RNRK GEKN GKGL RHFS MKVC EKVQ RKGT TSYN EVAD ELVA EFSA ADNH ILPN ESAY DQKN IRRR VYDA LNVL MAMN IISK EKKE IKWI GLPT NSAQ ECQN LEVE RQRR LERI KQKQ SQLQ ELIL QQIA FKNL VQRN RHAE QQAS RPPP PNSV IHLP FIIV NTSK KTVI DCSI SNDK FEYL FNFD NT
Gene ID:	7027
Uniprot:	Q14186
Cellular Location:	Nucleus
Calculated MW:	34kDa/45kDa
Observed MW:	45kDa

Synonyms:

TFDP1, DILC, DP1, DRTF1, Dp-1

Background:

This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.

UniProt Protein Function:	TFDP1: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis. Belongs to the E2F/DP family.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 13q34 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; protein domain specific binding; transcription factor activity; transcription factor binding Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009]
UniProt Code:	Q14186
NCBI GenInfo Identifier:	3122926
NCBI Gene ID:	7027
NCBI Accession:	Q14186.1
UniProt Secondary Accession:	Q14186,Q5JSB4, Q8IZL5, B4DLQ9,
UniProt Related Accession:	Q14186
Molecular Weight:	34,921 Da
NCBI Full Name:	Transcription factor Dp-1
NCBI Synonym Full Names:	transcription factor Dp-1
NCBI Official Symbol:	TFDP1
NCBI Official Synonym Symbols:	DP1; DILC; Dp-1; DRTF1
NCBI Protein Information:	transcription factor Dp-1
UniProt Protein Name:	Transcription factor Dp-1
UniProt Synonym Protein Names:	DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1
Protein Family:	Transcription factor
UniProt Gene Name:	TFDP1
UniProt Entry Name:	TFDP1_HUMAN

	Western blot analysis of extracts of various cell lines, using TFDP1 antibody (CAB5422) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon damage using TFDP1 antibody (CAB5422) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung using TFDP1 antibody (CAB5422) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-TFDP1 Antibody (CAB14789)	DP-1 Colorimetric Cell-Based ELISA Kit
Anti-DP1 Antibody (CAB5214)
TFDP1 Antibody (PACO01609)
TFDP1 Antibody (PACO12712)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)