Activator protein 2 (AP-2/TFAP2A) Rabbit Polyclonal Antibody (CAB0416)
The TFAP2A Polyclonal Antibody (CAB0416) is a valuable tool for research involving TFAP2A, a transcription factor that plays a crucial role in gene regulation and cellular differentiation processes. Raised in rabbits, this antibody demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the TFAP2A protein, this antibody enables precise detection and analysis in various cell types, making it ideal for studies in developmental biology, cancer research, and stem cell biology.
TFAP2A, also known as AP-2 alpha, is involved in the regulation of gene expression, cell growth, and apoptosis, making it a key player in the control of cellular processes. Its significance in various biological pathways makes it a promising target for research into developmental disorders, cancer progression, and stem cell differentiation. Understanding the function of TFAP2A is essential for unraveling its impact on different cellular processes and identifying potential therapeutic targets for related diseases.
Product Name:
Activator protein 2 (AP-2/TFAP2A) Rabbit Polyclonal Antibody
SKU:
CAB0416
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-200 of human Activator protein 2 (AP-2/TFAP2A) (NP_003211.1).
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha; Activator protein 2 (AP-2/TFAP2A)
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
50kDa
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7020
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Activator protein 2 (AP-2/TFAP2A) antibody (CAB0416) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
