The TET3 Monoclonal Antibody is a key tool for researchers studying TET3, an enzyme involved in DNA demethylation and gene regulation. This antibody, produced through hybridoma technology, has high specificity and sensitivity for detecting TET3 in various sample types, including human tissues. Validated for use in techniques such as immunofluorescence and immunoprecipitation, this antibody enables precise localization and quantification of TET3 in cells and tissues.TET3, a member of the TET family of proteins, plays a critical role in the dynamic regulation of DNA methylation, which is essential for processes such as embryonic development and maintenance of pluripotency.
Dysregulation of TET3 has been implicated in various diseases, including cancer and neurological disorders. By using the TET3 Monoclonal Antibody, researchers can further explore the function and regulatory mechanisms of TET3, shedding light on its potential role in disease pathogenesis and therapeutic targeting.
Product Name:
TET3 Monoclonal Antibody
SKU:
CAB21930
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of mouse TET3 (Q8BG87).
cytoplasm, female pronucleus, male pronucleus, nucleoplasm, nucleus
Calculated MW:
195kDa
Observed MW:
230kDa/
Enables methylcytosine dioxygenase activity. Involved in DNA demethylation of male pronucleus. Acts upstream of or within DNA demethylation. Located in cytoplasm; female pronucleus; and male pronucleus. Is expressed in several structures, including central nervous system; early conceptus; future brain; and genitourinary system. Orthologous to human TET3 (tet methylcytosine dioxygenase 3).
Purification Method:
Affinity purification
Gene ID:
194388
Clone Number:
ARC52663
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Neuro-2a cells, using TET3 Rabbit mAb (CAB21930) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
