The TDO2 Polyclonal Antibody (CAB13182) is a valuable tool for researchers studying tryptophan 2,3-dioxygenase (TDO2), an enzyme involved in the breakdown of the essential amino acid tryptophan. TDO2 plays a crucial role in the regulation of immune responses, inflammation, and neurotransmission.This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the TDO2 protein, researchers can analyze its expression levels and activity in various cell types, providing insights into its role in disease states such as cancer, autoimmune disorders, and neurological conditions.
Understanding the function of TDO2 is essential for developing targeted therapies that manipulate tryptophan metabolism to modulate immune responses and inflammation. The TDO2 Polyclonal Antibody is a valuable tool for uncovering the complexities of tryptophan catabolism and its impact on health and disease.
Product Name:
TDO2 Rabbit Polyclonal Antibody
SKU:
CAB13182
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TDO2 (NP_005642.1).
Sequence:
VLQN MRVP YNRR HYRD NF
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:100 - 1:500
Synonyms:
TO; TDO; TPH2; TRPO; HYPTRP; TDO2
Positive Sample:
HT-29,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
cytosol
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
Purification Method:
Affinity purification
Gene ID:
6999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TDO2 antibody (CAB13182) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
