The TDG Polyclonal Antibody (CAB5756) is a valuable tool for researchers studying TDG, a key enzyme involved in DNA demethylation processes. This rabbit-raised antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding specifically to the TDG protein, this antibody allows for the accurate detection and analysis of TDG expression levels in various cell types.TDG, or Thymine DNA Glycosylase, is essential for maintaining genomic integrity by removing aberrant or misincorporated thymine bases from DNA. Dysregulation of TDG activity has been implicated in various diseases, including cancer and neurodevelopmental disorders.
By studying TDG expression and function using this antibody, researchers can gain insights into its role in DNA repair, epigenetic regulation, and disease pathology.Overall, the TDG Polyclonal Antibody (CAB5756) is a valuable tool for investigating the biological functions of TDG and its potential implications in disease development. Its high specificity and sensitivity make it an excellent choice for researchers in the fields of genetics, epigenetics, and cancer biology.
Product Name:
TDG Rabbit Polyclonal Antibody
SKU:
CAB5756
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TDG (NP_003202.3).
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
Purification Method:
Affinity purification
Gene ID:
6996
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat thymus, using TDG antibody (CAB5756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
