The TCHP Antibody (PAC041242) is a high-quality polyclonal antibody designed for research involving the TCHP protein, a critical player in signaling pathways regulating cell growth and differentiation. This antibody is raised in rabbits and is specifically reactive with human samples, making it an ideal tool for studying TCHP in various biological contexts.TCHP, also known as trichoplein, is a key regulator of microtubule organization and centriole duplication, essential processes in cell division and maintenance of cellular structure. Its role in these pathways makes it an attractive target for research in cell biology, development, and cancer progression.
The TCHP Antibody binds specifically to the TCHP protein, enabling researchers to detect and analyze its expression levels in different cell types and experimental conditions.By studying the function of TCHP, researchers can gain valuable insights into the mechanisms underlying cell division, cell motility, and potentially identify novel therapeutic targets for cancer treatment and other diseases related to cell proliferation and differentiation. The TCHP Antibody is validated for use in Western blot applications, providing reliable results for researchers investigating the role of TCHP in their studies.
Immunohistochemistry of paraffin-embedded human skin tissue using PACO41242 at dilution of 1:100.
Immunofluorescence staining of MCF-7 cells with PACO41242 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO41242 at dilution of 1:100.
Background:
Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells. Is a negative regulator of ciliogenesis.
Synonyms:
Trichoplein keratin filament-binding protein (Protein TCHP) (Mitochondrial protein with oncostatic activity) (Mitostatin) (Tumor suppressor protein), TCHP
UniProt Protein Function:
TCHP: Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells. Belongs to the TCHP family.Protein type: Tumor suppressorChromosomal Location of Human Ortholog: 12q24.11Cellular Component: apical cortex; centrosome; cytoplasm; keratin filament; mitochondrion; plasma membraneMolecular Function: protein bindingBiological Process: apoptosis; negative regulation of cell growth
