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TCF7 Transcription Factor Activity Assay

SKU:
TFAB00132
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
TCF7
Reactivity:
Human
Mouse
Rat
$719
Frequently bought together:

Description

system_update_altDatasheet

TCF7 Transcription Factor Activity Assay

The TCF7 Transcription Factor Activity Assay Kit is a powerful tool for studying the activity of TCF7, an important transcription factor involved in key cellular processes. This kit allows for the accurate and sensitive detection of TCF7 activity in various biological samples, including cell lysates and tissue homogenates.TCF7 is known to play a critical role in cellular development, differentiation, and signal transduction pathways. Dysregulation of TCF7 activity has been implicated in various diseases, including cancer, inflammatory disorders, and developmental abnormalities.

Therefore, monitoring TCF7 activity can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high performance and versatility, the TCF7 Transcription Factor Activity Assay Kit is an essential tool for researchers studying transcriptional regulation and its impact on cellular physiology and disease pathogenesis. Trust in this kit for reliable and reproducible results to advance your research efforts.

Product Name:TCF7 Transcription Factor Activity Assay
Product Code:TFAB00132
Target:TCF7
Synonyms:T-cell-specific transcription factor 1, T-cell factor 1, TCF-1
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie TCF7 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:TCF7: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. By TCF7L2 and CTNNB1. Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. Belongs to the TCF/LEF family. 16 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function:protein binding; sequence-specific DNA binding; beta-catenin binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; transcription, DNA-dependent; alpha-beta T cell differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; embryonic digestive tract morphogenesis; generation of neurons; T cell receptor V(D)J recombination; immune response; brain development; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis

NCBI Summary:The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:P36402
NCBI GenInfo Identifier:209572716
NCBI Gene ID:6932
NCBI Accession:P36402.3
UniProt Secondary Accession:P36402,Q86WR9, Q9UKI4, B3KSH3,
UniProt Related Accession:P36402
Molecular Weight:384
NCBI Full Name:Transcription factor 7
NCBI Synonym Full Names:transcription factor 7 (T-cell specific, HMG-box)
NCBI Official Symbol:TCF7  
NCBI Official Synonym Symbols:TCF-1  
NCBI Protein Information:transcription factor 7; T-cell-specific transcription factor 1
UniProt Protein Name:Transcription factor 7
UniProt Synonym Protein Names:T-cell-specific transcription factor 1; T-cell factor 1; TCF-1
Protein Family:Transcription factor
UniProt Gene Name:TCF7  
UniProt Entry Name:TCF7_HUMAN
Antibodies
Anti-TCF7 Antibody (CAB3091)
TCF7 Antibody (PACO01596)
TCF7 Antibody (PACO18446)

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