The TCF7 Polyclonal Antibody (CAB3091) is a research tool specifically designed for studying TCF7, a transcription factor involved in various cellular processes such as cell proliferation, differentiation, and development. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.TCF7, also known as T-cell factor 1, is a key player in the Wnt signaling pathway and is essential for regulating gene expression in T cells and other cell types. By targeting the TCF7 protein, this antibody enables researchers to detect and analyze TCF7 expression in different cell types, making it ideal for studies in immunology, stem cell biology, and cancer research.
Understanding the role of TCF7 in cell signaling and gene regulation is crucial for unraveling its involvement in diseases such as cancer, autoimmune disorders, and developmental abnormalities. The TCF7 Polyclonal Antibody provides researchers with a valuable tool for investigating the function of TCF7 and its potential as a therapeutic target in various pathological conditions.
Product Name:
TCF7 Rabbit Polyclonal Antibody
SKU:
CAB3091
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCF1/TCF7 (NP_003193.2).
This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling.
Purification Method:
Affinity purification
Gene ID:
6932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TCF1/TCF7 antibody (CAB3091) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
