The TCF3 Polyclonal Antibody (CAB20013) is a valuable tool for researchers studying the TCF3 protein, a transcription factor involved in the regulation of gene expression and cell differentiation. This antibody, produced in rabbits, exhibits a high level of reactivity with human samples and has been validated for use in Western blot applications.The TCF3 antibody specifically binds to the TCF3 protein, allowing for its detection and analysis in a variety of cell types. This makes it ideal for research in the fields of molecular biology and developmental biology, as TCF3 plays a critical role in the control of cell fate and development.
Furthermore, TCF3 is known to be dysregulated in a variety of diseases, including cancers and developmental disorders. By investigating the function and expression of TCF3 using this polyclonal antibody, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
Product Name:
TCF3 Rabbit Polyclonal Antibody
SKU:
CAB20013
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-525 of human TCF3 (NP_003191.1).
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Purification Method:
Affinity purification
Gene ID:
6929
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TCF3 antibody (CAB20013) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
