The TCF12 Polyclonal Antibody (CAB4146) is a valuable tool for researchers studying TCF12, a transcription factor involved in regulating gene expression and cell development. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the TCF12 protein, it enables precise detection and analysis in various cell types, making it an ideal choice for studies in developmental biology, stem cell research, and cancer biology.
TCF12, also known as Transcription factor 12, is a key player in controlling gene expression and cellular differentiation processes. Its role in regulating cell fate decisions and development makes it a promising target for investigations into embryonic development, tissue regeneration, and disease progression. Understanding the functions of TCF12 is essential for unraveling the mechanisms underlying cellular processes and developing therapeutic strategies targeted at these pathways.
Product Name:
TCF12 Rabbit Polyclonal Antibody
SKU:
CAB4146
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 443-682 of human TCF12 (NP_003196.1).
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
6938
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of Jurkat, using TCF12 antibody (CAB4146) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
