The TCEA3 Polyclonal Antibody (PAC059904) is a valuable tool for researchers studying TCEA3, a protein involved in transcription regulation. This antibody, generated in rabbits, shows high reactivity with human samples and is suitable for Western blot applications. By binding specifically to TCEA3, this antibody allows for the detection and analysis of the protein in various cell types, making it a versatile option for studies in molecular biology and gene expression.TCEA3 is known for its role in RNA polymerase II-mediated transcription and has been linked to gene regulation mechanisms.
Its involvement in the control of gene expression makes it an important target for research in fields like cancer biology, developmental biology, and epigenetics. Investigating the function of TCEA3 can provide valuable insights into the mechanisms underlying gene transcription and potential therapeutic strategies for diseases related to gene dysregulation.
IHC image of PACO59904 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO59904 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Synonyms:
Transcription elongation factor A protein 3 (Transcription elongation factor S-II protein 3) (Transcription elongation factor TFIIS.h), TCEA3, TFIISH
UniProt Protein Function:
TCEA3: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Belongs to the TFS-II family.Chromosomal Location of Human Ortholog: 1p36.12
