Anti-TCAF2 Antibody (PACO60689)

Antibody Name:	TCAF2 Antibody (PACO60689)
Antibody SKU:	PACO60689
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human TRPM8 channel-associated factor 2 protein (472-590AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO60689 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of Hela cells with PACO60689 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO60689 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Isoform 2: Negatively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration stimulation in a TRPM8-dependent manner.
Synonyms:	TRPM8 channel-associated factor 2 (TRP channel-associated factor 2), TCAF2, FAM115C FAM139A

UniProt Protein Function:	Isoform 2: Negatively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration stimulation in a TRPM8-dependent manner.
UniProt Protein Details:
NCBI Summary:
UniProt Code:	A6NFQ2
NCBI GenInfo Identifier:	194097428
NCBI Gene ID:	285966
NCBI Accession:	NP_001123497.1
UniProt Secondary Accession:	A6NFQ2,Q14D25, Q17RQ4, Q8IWQ0, Q8NF84, B4DK02
UniProt Related Accession:	A6NFQ2
Molecular Weight:	92,008 Da
NCBI Full Name:
NCBI Synonym Full Names:	TRPM8 channel associated factor 2
NCBI Official Symbol:	TCAF2
NCBI Official Synonym Symbols:	GATD9; FAM115C; FAM139A
NCBI Protein Information:	TRPM8 channel-associated factor 2
UniProt Protein Name:	TRPM8 channel-associated factor 2
UniProt Synonym Protein Names:	TRP channel-associated factor 21 PublicationManual assertion based on opinion iniRef.7"TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity."Gkika D., Lemonnier L., Shapovalov G., Gordienko D., Poux C., Bernardini M., Bokhobza A., Bidaux G., Degerny C., Verreman K., Guarmit B., Benahmed M., de Launoit Y., Bindels R.J., Fiorio Pla A., Prevarskaya N.J. Cell Biol. 208:89-107(2015) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION (ISOFORM 2), INTERACTION WITH TRPM8 AND TRPV6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Protein Family:	TRPM8 channel-associated factor
UniProt Gene Name:	TCAF2
UniProt Entry Name:

Secondary Antibody

Biological Role of GLP-1