TC(Total cholesterol) ELISA Kit (UNFI0113)
- SKU:
- UNFI0113
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 9.375ng/ml
- Range:
- 15.625-1000ng/ml
Frequently bought together:
Description
Product Name: | TC(Total cholesterol) ELISA Kit |
Alias: | TC ELISA Kit, Total cholesterol ELISA Kit |
SKU: | UNFI0113 |
Size: | 96T |
Species: | Universal |
Sample Type: | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
Detection Method: | Competitive ELISA, Coated with Antigen |
Detection Wavelength: | OD450 |
Reaction Duration: | 2 hours |
Range: | 15.625-1000ng/ml |
Sensitivity: | 9.375ng/ml |
Storage: | 2-8°C(Sealed), Don't cryopreserve. |
Specificity: | Specifically binds with TC, no obvious cross reaction with other analogues. |
Component | Size (96T) | Storage |
ELISA Microplate (Dismountable) | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C |
Lyophilized Standard | 2 vials | Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C |
Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 2-8°C (Avoid Direct Light) |
HRP-Streptavidin Conjugate (SABC, 100X) | 120 ul | |
TMB Substrate | 10 ml | |
Sample Dilution Buffer | 20 ml | 2-8°C |
Antibody Dilution Buffer | 10 ml | |
SABC Dilution Buffer | 10 ml | |
Stop Solution | 10 ml | |
Wash Buffer (Concentrated, 25X) | 30 ml | |
Plate Sealer | 5 pieces | |
Product Description | 1 copy |
Other materials and equipment required:
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommended.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips (Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
Recovery: | Add a certain amount of TC into the sample. Calculate the recovery by comparing the measured value with the expected amount of TC in the sample. | ||||||||||||||||||||||||||||||||||||||||||
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Linearity: | Dilute the sample with a certain amount of TC at 1:2, 1:4 and 1:8 to get the recovery range. | ||||||||||||||||||||||||||||||||||||||||||
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Precision(%): |
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Stability: | Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data. | ||||||||||||||||||||||||||||||||||||||||||
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Step 1: | Wash the plate twice before adding the standard and sample. |
Step 2: | Add 50ul standard or sample into each well. Immediately add 50ul biotin-labeled antibody working solution into each well (The tip can't be reused after touching the liquid.), gently shake the plate for 1min, seal closely and statically incubate for 45 minutes at 37°C. |
Washing: Wash the plate three times and immerse for 1min each time. | |
Step 3: | Add 100ul HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37°C. |
Washing: Wash the plate five times and immerse for 1min each time. | |
Step 4: | Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.) |
Step 5: | Add 50ul stop solution. Read at 450nm immediately and calculate. |